Report #: 7-71
Date: May 14, 2007

EXECUTIVE SUMMARY

Adya® Mineral solution manufactured by Primordial Solutions, Inc. was tested for antibacterial properties.
Four control batches were prepared with four different types of Coliform bacteria in sterilized water and
tested for Total Coliform/E. coli using the m-ColiBlue-24 method. Four similar Coliform batches were
prepared and treated with the Adya® Mineral solution in a 1:1000 proportion and tested again using the
same Coliform method. Coliform bacteria were present in the control batches but absent in the treated
batches.

INTRODUCTION

Adya® Mineral solution manufactured by Primordial Solutions, Inc. was tested for antibacterial properties.
Four control batches were prepared with four different types of Coliform bacteria in sterilized water and
tested for Total Coliform/E. coli using the m-ColiBlue-24 method. The four type of bacteria used were
Escherichia coli, Enterobacter cloacae, Proteus mirablis, and Pseudomonas aeuginosa. Four similar
Coliform batches were prepared and treated with the Adya® Mineral solution in a 1:1000 proportion and
tested again using the same Coliform method. Coliform bacteria were present in the control batches but
absent in the treated batches.

MATERIALS

Adya® Mineral concentrated Solution

PotableWatr Coliform ERA Catalog # 694 containing Escherichia coli, Enterobacter cloacae, Proteus
mirablis, and Pseudomonas aeuginosa at about 500 colonies forming units/mL (CFU/mL)

PROCEDURE

1.     Remove the vials from the refrigerator and allow them to warm to room temperature.

2.     Place the sterilized water containers in the incubator at 35 to 37°C for approximately 30 minutes.

3.     After the 30-minute warming period, remove one sterilized water container from the incubator.

4.     Carefully open the sterilized water container.

5.     Open the appropriate bacteria sample vial and aseptically transfer the gelatin tablet into the

sterilized water container and labeled accordingly.

6.     Properly dispose the empty glass vial and pouch.

7.     Reseal the sterilized water container that now contains the bacteria sample.

8.     Prepared all the samples as above.

9.     Place the inoculated samples into the incubator at 35 to 37°C for an additional 30 minutes to

dissolve the samples.

11.   Prepare the filter units and filter the first four samples as per method mColiBlue-24 (these are the
control samples).

12.   Place the filter membrane in the Petri dish and incubate for 24 hours at 35 to 37°C.

RESULTS

Parameter	Control Samples	Adya Mineral Solution	EPA MCL
Escherichia coli	Present	Absent	Absent
Enterobacter cloacae	Present	Absent	Absent
Proteus mirablis	Present	Absent	Absent
Pseudomonas aeuginosa	Present	Absent	Absent

CONCLUSION

The Adya® Mineral solution prepared at 1:1000 effectively kills the four types of bacteria present in the
challenge water at about 500 CFU/mL.

Jaime A. Young
Lab Director